Life Sciences

“Chance favours only the prepared minds”

Advances made in Plant Sciences, Animal Sciences and other disciplines of life sciences have given birth to a new frontier branch i.e. “Biotechnology”. Biotechnology has evolved as a link between Biological Sciences and Technology. The two most important and immediate requirements of society are good food and health besides residential and other commercial requirements. Biotechnology and Microbiology is going to make substantial contribution in solving both health and food problems. The biochemical and biological processes of living organisms helps in making modified products for betterment of living being. The demand of these courses is increasing day by day in every sphere such as pharmaceutical, agriculture and medical sciences. Application of Biotechnology and Microbiology in pharmaceutical industries is currently used in the production of proteins such as Insulin, Interferon, Antibiotics, Monoclonal Antibodies and designing vaccine for bacterial, viral and other parasitic diseases.

Life Science Labs

There are dedicated labs of Biotechnology, Microbiology, Molecular Biology, Plant tissue culture, Immunology and Enzymology. College of Life Sciences is structured with highly advanced equipments on which student gets hands on demonstration like PCR (Thermalcycler), Spectrophotometer, Micro-centrifuge/Cooling Compufuge, Trans-illuminator, ELISA, Haemo-cytometer, Electrophoresis Unit, Binocular and Monocular Microscope, Compound and Dissecting Microscope, Hot Air Oven, Laminar Air Flow, Incubator, pH Meter, Magnetic Stirrer, Shaker with Water Bath, Colony Counter, Distillation Plant, Vacuum Pump, Vortex Mixer, Refrigerator, Deep freezer, Electronic and Physical Balance, Colorimeter, Camera Lucida, Micrometer, Viscometer, Stalagmometer, Pyknometer, Ganog’s Potometer, Distillation Plant and so on. Students are requisite to handle all these instruments while experiments. The Labs are equipped with equipment of international standards.

Careers in Life Sciences

Both Biotechnology and Microbiology go hand in hand. It can grow your future with scientific evaluation and expertise. After completion of the curriculum in these branches you can look forward to::

Research Organization Pharmaceutical Industry Food & Beverage Industry
Paper & Rubber Industry Leather & Tanning Industry Textile Industry
As a-
Research Scientist Molecular Biologist Cell Biologist
Quality Control Officer Immunologist Nano Biotechnologist
Environmental Bio-technologist Forensic Expert Project Assistant
JRF Drug Designer

Practical Expertise for M.Sc. Biotechnology Course

Genetic Engineering: Isolation of RNA and DNA, Restriction enzyme digestion, Ligation, Competent cell preparation, Transformation and selection of recombinants, Molecular size determination of DNA, Maintenance and preservation of Recombinant Clones, Polymerase Chain Reaction (PCR), Southern and Western blotting.

Bioinformatics: Amino acid and Nucleotide sequencing and ORF Analysis using BLASTA & FASTA and relevant internet surfing.

Tissue Culture: Experiments on cell cultures, Haploid plant production, Protoplast isolation, Callus Culture, Stages of mitosis.

Bio-processing: Production of alcohol, Strain improvement, Fermentation mechanism.

Immunology: Preparation of antigen hyperimmune sera, Gel diffusion, Counter immuno electrophoresis, dot ELISA, WIDAL, VDRL, Western blotting.

Biochemistry: Quantitative analysis of Carbohydrates, Proteins, Acid value, Beer Lambert’s Law, Paper chromatography, Thin layer chromatography, Enzymatic assays, Cell immobilization, Protein precipitation, Casein isolation, pKa of amino acids, Protein purification.

Microbiology: Media preparation, Plating and Streaking techniques, Serial dilution, Gram staining, Spore, Capsular and Acid-fast staining, Fungal staining, Antibiotic sensitivity and MIC, Growth curve, Hanging drop, Maintenance and Preservation of microbial cultures.

Practical Expertise for B.Sc. Biotechnology/Microbiology Course

Biotechnology: Qualitative analysis of proteins, carbohydrates, nucleic acids, Paper and TLC, protein precipitation, concentration and estimation, standard curve preparation and quantitative analysis of proteins, carbohydrates and DNA, Media preparation & stock, stab, spread, steak and pour plating, Staining of bacteria, fungi, spores, Identification of microorganism, Serial dilution, Isolation of microbes from air, soil & water, Antibiotic sensitivity, Antigen preparation, Immunization, Radial & double immuno diffusion, ELISA, Leucocytes identification and counting by TLC and DLC, Haemagglutinations, Cell Size determination, Haemo-cytometer, Determination of blood groups, WIDAL, Bioreactor operation, Plant Tissue Culture, DNA Isolation and Agarose Gel Electrophoresis.

Microbiology: Various sterilization and disinfection procedures, Media preparation, isolation and purification of microorganisms from environmental samples, Serial dilution techniques and colony count, MPN of water, Paper and thin layer chromatography, RBC count by Haemo-cytometer, Testing of blood groups, Biochemical tests for identification of members of enterobacteriaceae (IMVIC Test etc), Titration of antigen and antibody by various Immunological techniques.

Botany: Biochemical analysis of Plant Tissues, Soil analysis, Experiments in Ecological adaptations, Plant physiology, Plant genetics, Stages of mitosis in Plant Tissues, Study of representative Plants of different families both morphology and anatomy, section cutting, slide preparation and staining by various dyes.

Chemistry: Qualitative analysis of organic and inorganic mixtures, Gravimetric estimation, Titrations, Determination of melting point, boiling point, solubility, Preparation of various organic compounds and derivatives, transition temperature and critical temperature of compounds, Chromatographic separation, Conductometric & Colorimetric determination techniques of separation i.e. Distillation, Crystallization, Sublimation, Viscosity & Surface tension of liquids.